An investigation into 3D printing of osteological remains: the metrology and ethics of virtual anthropology

Three-dimensional (3D) printed human remains are being utilised in courtroom demonstrations of evidence within the UK criminal justice system. This presents a potential issue given that the use of 3D replicas has not yet been empirically tested or validated for use in crime reconstructions. Further, recent movements to critically evaluate the ethics surrounding the presentation of human remains have failed to address the use of 3D printed replica bones. As such, this research addresses the knowledge gap surrounding the accuracy of 3D printed replicas of skeletal elements and investigates how the public feels about the use of 3D printed replicas. Three experimental studies focussed on metrology and identified 3D printed replicas to be accurate to within ± 2.0 mm using computed tomography (CT) scanning, and to within ± 0.2 mm or to 0-5% difference using micro-CT. The potential loss of micromorphological details was also examined and identified that quality control steps were key in identifying and mitigating loss of detail. A fourth experimental study collected data on the opinion of the public of the use of 3D printed human remains in courtroom demonstrations. Respondents were broadly positive and considered that prints can be produced ethically by maintaining the dignity and respect of the decedent. A framework that helps to assess ethical practices was developed as well as an adaptable pathway that can assist with assessing the quality and accuracy of 3D prints. The findings from this research contribute to an empirical evidence base that can underpin future 3D printed crime reconstructions and provides guidance for creating accurate 3D prints that can inform future practice and research endeavours

Imaging in forensic science: Five years on

The Journal of Forensic Radiology and Imaging was launched in 2013 with the aim to collate the literature and demonstrate high-quality case studies on image-based modalities across the forensic sciences. Largely, the focus of this journal has been on the transmissive aspect of forensic imaging, and therefore a significant number of high-quality case studies have been published focusing on computed tomography and magnetic resonance imaging. As a result, the ‘and imaging’ aspect is often neglected. Since 2013, technology has fundamentally evolved, and a number of new techniques have become accessible or have been demonstrated as particularly useful within many sub-disciplines of forensic science. These include active and passive surface scanning techniques, and the availability of three-dimensional printing. Therefore, this article discusses non-contact techniques, their applications, advantages, and considerations on the current state of play of imaging in forensic science

Drilling down into ethics: A thematic review of ethical considerations for the creation and use of 3D printed human remains in crime reconstruction

The existing literature contains some exploration of the ethics concerning human remains in forensic and virtual anthropology. However, previous work has stopped short of interrogating the underlying ethical concepts. The question of how people understand and apply these concepts in practice, and what it means to act ethically, remain underexplored. This thematic review explores the ethical considerations that contribute to the creation and use of 3D printed human remains for forensic purposes. The three main branches of ethical theory are outlined to explore how they may apply to forensic practice. Key themes relating to 3D printing human remains in forensic contexts were explored to better understand the ethics landscape, ethical challenges, and the current guidelines in place. Through this thematic review, nine ethics principles were identified as key principles for guiding best practice: anonymity, autonomy, beneficence, consent, context, justice, non-maleficence, proportionality, and transparency. It is suggested that these principles could be incorporated into adaptable guidelines going forward to support ethical practice. The findings also suggest that holistic ethics cognition training may have value in supporting forensic scientists in ethical decision-making, together with procedural and structural design that may promote best practice and reduce cognitive load

An overview of 3D printing in forensic science: the tangible third-dimension

There has been a rapid development and utilization of three‐dimensional (3D) printing technologies in engineering, health care, and dentistry. Like many technologies in overlapping disciplines, these techniques have proved to be useful and hence incorporated into the forensic sciences. Therefore, this paper describes how the potential of using 3D printing is being recognized within the various sub‐disciplines of forensic science and suggests areas for future applications. For instance, the application can create a permanent record of an object or scene that can be used as demonstrative evidence, preserving the integrity of the actual object or scene. Likewise, 3D printing can help with the visualization of evidential spatial relationships within a scene and increase the understanding of complex terminology within a courtroom. However, while the application of 3D printing to forensic science is beneficial, currently there is limited research demonstrated in the literature and a lack of reporting skewing the visibility of the applications. Therefore, this article highlights the need to create good practice for 3D printing across the forensic science process, the need to develop accurate and admissible 3D printed models while exploring the techniques, accuracy and bias within the courtroom, and calls for the alignment of future research and agendas perhaps in the form of a specialist working group

Sex estimation from virtual models: exploring the potential of stereolithic 3D crania models for morphoscopic trait scoring

UNLABELLED: Modern computed tomography (CT) databases are becoming an accepted resource for the practice and development of identification methods in forensic anthropology. However, the utility of 3D models created using free and open-source visualization software such as 3D Slicer has not yet been thoroughly assessed for morphoscopic biological profiling methods where virtual methods of analysis are becoming more common. This paper presents a study that builds on the initial findings from Robles et al. (2020) to determine the feasibility of estimating sex on stereolithic (STL) 3D cranial models produced from CT scans from a modern, living UK population (n = 80) using equation 2 from the Walker's (2008) morphoscopic method. Kendall's coefficients of concordance (KCC) indicated substantial agreement using cranial features scores in an inter-observer test and a video-inter-observer test. Fleiss' Kappa scores showed moderate agreement (0.50) overall between inter-observer sex estimations, and for observer sex estimations in comparison to recorded sexes (0.56). It was found that novice users could virtually employ morphoscopic sex estimation methods effectively on STL 3D cranial models from modern individuals. This study also highlights the potential that digital databases of modern living populations can offer forensic anthropology. KEY POINTS: First example of Walker's (2008) method applied to a living UK population.Open-source software is a valuable resource for crime reconstruction approaches.Male scoring bias was observed in method application.Forensic anthropologists would benefit from virtual anthropology training to use and interpret 3D models.Digital databases offer more ethical, diverse, modern populations for future research

A multi-method assessment of 3D printed micromorphological osteological features

The evaluation of 3D printed osteological materials has highlighted the difficulties associated with accurately representing fine surface details on printed bones. Moreover, there is an increasing need for reconstructions to be demonstrably accurate and reliable for use in the criminal justice system. The aim of this study was to assess the surface quality of 3D prints (n = 9) that presented with micromorphological alterations from trauma, taphonomy and pathology processes. The archaeological bones were imaged using micro-CT scanning and 3D printed with selective laser sintering (SLS) printing. A multi-method experimental approach subsequently identified: (1) the 3D printed bones to be metrically accurate to within 1.0 mm; (2) good representation of micromorphological surface features overall, albeit with some loss of intricate details, depths, and fine textures that can be important for visual processing; (3) five of the nine 3D printed bones were quantitatively scored as accurate using the visual comparison method; and, (4) low mesh comparison distances (± 0.2 mm) between the original models and the digitised 3D print models. The findings offer empirical data that can be used to underpin 3D printed reconstructions of exhibits for use in courts of law. In addition, an adaptable pathway was presented that can be used to assess 3D print accuracy in future reconstructions

Sex estimation from virtual models: Exploring the potential of stereolithic (STL) 3D crania models for morphoscopic trait scoring

Modern computed tomography (CT) databases are becoming an accepted resource for the practice and development of identification methods in forensic anthropology. However, the utility of 3D models created using free and open-source visualisation software such as 3D Slicer have not yet been thoroughly assessed for morphoscopic biological profiling methods where virtual methods of analysis are becoming more common. This paper presents a study that builds on the initial findings from Robles et al. 2020 to determine the feasibility of estimating sex on STL 3D cranial models produced from CT scans from a modern, living UK population (n=80) using equation 2 from the Walker (2008) morphoscopic method. Kendall's coefficients of concordance (KCC) indicated substantial agreement using cranial features scores in an inter-observer test and a video-inter-observer test. Fleiss's kappa scores showed moderate agreement (0.50) overall between inter-observer sex estimations, and for observer sex estimations in comparison to recorded sexes (0.56). It was found that novice users could virtually employ morphoscopic sex estimation methods effectively on STL 3D cranial models from modern individuals. This study also highlights the potential that digital databases of modern living populations can offer forensic anthropology

Accuracy of computed radiography in osteometry: a comparison of digital imaging techniques and the effect of magnification

Introduction Osteometric data may be obtained using digital imaging techniques, such as post-mortem computed tomography (PMCT) and digital radiography, non-invasively and without ethical objection. Osteometric data from PMCT is known to be as accurate as taking direct measurements. Digital radiography is more accessible and affordable than PMCT but is limited due to the magnification of its subjects. Objectives To investigate the viability of implementing digital radiographic techniques for measurement of long bones, and to establish whether magnification can be accurately corrected for. Materials and Methods Twenty hind pig (Sus scrofa) legs were imaged using computed radiography (CR) and PMCT, and osteometric data obtained from the digital images and 3D CT volume reconstructions. Direct measurements were taken following maceration. A calibration object was imaged using CR, to provide magnification correction factors. Results Accuracy was determined by mean absolute error (AE), giving values of 3.3 ± 2.5 mm for PMCT (MPR), 2.4 ± 1.3 mm for PMCT (3D), 11.1 ± 7.4 mm for CR (PA), and 18.3 ± 14.5 mm for CR (LAT). PMCT data was more accurate than CR data. Through applying correction for magnification, CR data became closer to the direct measurement data, and stature estimation was substantially altered. Conclusion Magnification in computed radiography affects osteometric data and resulting stature estimations. Applying correction factors may be a viable option for improving accuracy. For digital radiography to be used reliably in forensic anthropology, further empirical research is needed to validate a magnification correction method